Designing better enzymes: Insights from directed evolution

How enzymes adapt: lessons from directed evolution.

Enzymes that are adapted to widely different temperature niches are being used to investigate the molecular basis of protein stability and enzyme function. However, natural evolution is complex: random noise, historical accidents and ignorance of the selection pressures at work during adaptation all cloud comparative studies. Here, we review how adaptation in the laboratory by directed evolutio...

Designing a “better” brain: insights from experts and savants

1 Department of Psychological Sciences, University of Liverpool, Liverpool, UK 2 Centre for the Mind, School of Medicine, University of Sydney, Sydney, NSW, Australia 3 Centre for Research in Complex Systems, Faculty of Business, Charles Sturt University, Bathurst, NSW, Australia 4 Complex Systems Research Group, Faculty of Engineering and IT, University of Sydney, Sydney, NSW, Australia *Corre...

Directed evolution of industrial enzymes.

Directed evolution of glycosylating enzymes

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CADEE: Computer-Aided Directed Evolution of Enzymes

The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of...